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Company | Johnson & Johnson |
Address | Cambridge, MA, United States |
Employment type | FULL_TIME |
Salary | |
Category | Hospitals and Health Care |
Expires | 2023-05-29 |
Posted at | 1 year ago |
- Train new hires and mentor other scientists, contractors, and co-op students
- Design and perform on display-based technologies for antibody optimization, with an emphasis on phage and yeast display techniques.
- Manage laboratory operations including such as reviewing of experimental protocols and equipment manuals, writing protocols, ordering of reagents and supplies, inventory management, instrument QC, scheduling of equipment maintenance, safety inspections
- Design and clone antibody and protein based molecular entities
- Perform and expand established assays as well as develop and optimize new assays. Responsibilities will include cloning, plasmid scale-up, plasmid QC, cell culture, reagent preparation, E. coli and mammalian cell culture, ELISA and other binding assays and flow cytometry.
- Support the broad protein engineering efforts in the modality agnostic Therapeutics Discovery organization
- A minimum of 3 years of relevant laboratory experience is required
- Be able to independently process data, interpret results, update the database, and present results to applicable functional groups.
- MSc or PhD or equivalent in Molecular Biology, Biochemistry, Biotechnology, Biomedical Engineering, structural biology or related field is required
- Strong written and oral communication skills are required
- In-depth understanding and experience of antibody engineering is required
- Proficient knowledge of cellular/molecular biology, common biology laboratory assays such as ELISA, Luminex, and biolayer interferometry (Octet) and other assays are required
- Demonstrated experience working in a cross-functional organization is required
- Innovative approach and ready to step out of the box for creating and exploring new ideas with calculated risk taking is required
- Background in managing incoming requests, conducting design and inventory registration of plasmids in a central database system is preferred
- Experience in molecular biology techniques including bi-, multi-specifics, scFv, and/or chimeric antigen receptor (CAR) construct design and cloning is preferred
- Maintain accurate inventory of generated plasmids, lab notebooks, and other records and writing of technical reports, summaries, protocols, and manuscripts are preferred
- Experience with protein production using transient transfection/stable cell line generation using CHO and HEK-293 is preferred
- Experience in display-based technologies is preferred
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