Scientist, Molecular Biology Jobs

Company Overview

GC Therapeutics is the first genome-wide cell landscape exploration company using an integrated synthetic biology and AI-driven platform for cell programming. Its patent-pending and proprietary pluripotent stem cell differentiation technology platform TFomeTM was developed in Professor George Church’s lab, a pioneer in synthetic biology, and allows for the development of unique cell therapies with significantly streamlined manufacturing, improved cell quality, efficiency and speed. GC Therapeutics is based in Cambridge, MA. For additional information, please visit www.gc-tx.com.

Job Purpose

GC Therapeutics is seeking a talented and highly motivated Scientist for our Discovery team. The candidate will have strong expertise in molecular cloning, next generation sequencing (NGS), and high throughput screening (HTS). Reporting to the Head of Discovery Platforms, this individual will engineer novel cell therapies derived from human induced pluripotent stem cells (iPSCs) for regenerative and cell-based therapies. This role will involve bringing a new class of cellular therapies to patients using GCTx’s Human TFomeTM platform, a synthetic biology-driven technology to differentiate human stem cells. The scientist will be responsible for daily tasks involving the design and execution of experiments using large scale genomic libraries in iPSCs and analyzing their NGS readouts.

Duties and Responsibilities

General Laboratory Roles:

• Follow all safe laboratory practices and company policies.
• Directions.
• Work with other members of the research team and within GCTx to accomplish company goals.
• Responsible for experimental design, data generation, analysis, and communication of results to supervisor, and team members.
• Exemplify scientific curiosity and deep expertise with relevant literature and cutting-edge technologies to advance research
• Provide scientific and technical supervision to junior staff or contract research organizations (CRO) for defined projects.
• Maintain a detailed laboratory journal, summarize results and create presentations for internal group meetings.

Experimental Techniques:

Molecular Biology

• Utilize techniques such as PCR, molecular cloning and plasmid isolation.
• And single cell RNA-seq) and other gene expression or genomics analysis techniques.
• Design and validate nucleic acid-based libraries for high throughput screening purposes.
• Generate read outs from high-throughput screens using next-generation sequencing methods (e.g. library preparation for bulk

Cellular Biology

• Design and execute synthetic biology-based differentiation experiments of pluripotent stem cells.
• Culture PSC lines including maintenance and analysis (e.g. cell culture, transfection, preparation of media, passaging,
• Cryopreservation).
• Analyze differentiated cell products and establish QC metrics.

Qualifications

Essential Qualifications:

• Required experience with molecular cloning, high throughput screening and NGS.
• Effective communicator with excellent interpersonal skills.
• Strong work ethic with the ability to perform tasks independently and lead junior staff effectively.
• Strong project management and organizational skills and ability to prioritize and multitask.
• Proven experience in basic molecular biology laboratory techniques.
• Ability to work in a laboratory environment approximately 75% of the time including occasional weekend hours.
• Ph.D. degree in synthetic biology, cell biology, bioengineering, biochemistry, or related discipline and 5+ years of laboratory work experience in academia or industry.
• Proficient in analysis, presentation and documentation of scientific data and scientific reports.

Additional Preferred Qualifications:

• Experience with pluripotent stem cells (e.g. hiPSCs).
• Ability to analyze next generation sequencing or other genome-scale datasets.
• Experience in genome editing.
• Knowledge of R, Python and FlowJo.

Equal Opportunity Workplace: GC Therapeutics is an equal opportunity employer. We provide equal employment opportunities to all applicants for employment and existing employees without regard to ancestry, national origin, place of birth, race, color, gender, sexual orientation, marital status, pregnancy, religion, age, disability, gender identity, results of genetic testing, service in the military or otherwise to the full extent of all federal, state and local laws. GC Therapeutics’ equal employment opportunity policy applies to all terms and conditions of recruiting, hiring, placement, training, compensation, transfer, leave of absence, employment, promotion, layoff and termination of employment.