Associate Scientist Jobs

“This Company requires all staff, staff augmentation external workers, and certain other external workers, in the United States and Puerto Rico vaccinated from COVID 19 as a condition of assignment at this company. In accordance with applicable law, this company will provide reasonable accommodations staff augmentation external workers qualify on the basis of a medical reason or a sincerely held religious belief, practice, or observance. Such accommodation may not pose an undue hardship this company, its operations, or its staff.”

Top 3 Must Have Skill Sets

• Preferred skills include familiarity with automated chromatography hardware/software (e.g. AKTA) and previous experience culturing mammalian cells.
• Strong written and oral communication skills
• AS, BA or BS in science-related field + 2 years laboratory experience with significant hands-on laboratory work demonstrating mastery of micro-pipetting (single and multi-channel), sample manipulation in small tubes (microcentrifuge) and multi-well plates, SDS-PAGE; proficient using other basic lab equipment (e.g., pH meter, balances, centrifuges); making basic laboratory calculations, (e.g., for making standard buffers from dry chemical ingredients or liquid stock solutions), strong fundamental training in sterile technique and knowledge of safeguards for handling mammalian cultures.
• Ability to follow established laboratory protocols and adhere to data standards; attention to detail and careful execution of laboratory tasks are essential to our team

Day To Day Responsibilities

• Culturing mammalian cells such as HEK293 and/or Expi293
• Inventory Management and general routine laboratory activities
• Membrane Protein Purification
• Organize and set up -80C storage DNA inventories
• Register protein lots into/out of internal storage system, as needed
• Keeping records of data and procedures
• Use HPLC for protein quality control
• Perform batch affinity chromatography using anti-FLAG, Strep-Tactin, and Ni-NTA His affinity resin
• Use of AKTA chromatography instrumentation for protein purification
• Protein expression
• Measuring cell viability using Vi-cell
• Check stocks of required consumables and order and stock when necessary (i.e. common reagents)
• Running and imaging SDS-PAGE gels and western blots
• Use of spectrophotometry and colorimetric assays for concentration determination
• Cell lysis and membrane pellet preparation
• Running and imaging SDS-PAGE gels and western blots

PAY RATE : $28-$32/hr on W2

"This posting is for Contingent Worker, not an FTE"