Associate Scientist Jobs

Position Summary

We are seeking an experienced lab-based Associate Scientist / Senior Associate Scientist with expertise in molecular biology and assay development. The successful applicant will embrace the hands-on ethos of a start-up company, provide critical support for combinatorial cloning efforts, and generate in vitro data to validate our therapeutic platform. We seek intellectually curious candidates who take a data-driven and rigorous approach to solving problems. Our work is team-based, and this role requires extensive collaboration with colleagues in therapeutic and platform development teams.

Job Responsibilities

Reporting to the Head of Computational and Molecular Biology, the incumbent will be responsible for the design, execution, troubleshooting, and optimization of molecular biology assays, including:

·Generating and maintaining DNA construct libraries for test article production

·Performing high-throughput cloning of plasmid constructs with Golden Gate assembly and conventional cloning, and oversee sequence verification by external vendors

·Leading Next Generation Sequencing (NGS) library preparation for RNA-seq, DNA-seq, 10x Genomics single-cell assays

·Screening of high-throughput plasmid and viral libraries in mammalian lines and primary cell cultures

·Facilitate design of plasmid constructs using DNA software

Additional responsibilities include:

·Organization and tracking of DNA constructs and associated sequencing data across multiple projects.

·Collaboration with teammates to execute project plans, present data and recommend next steps.

·Authorship of research reports and relevant sections of regulatory documents to support first-in-human studies and contribute to intellectual property filings.

·Documentation of research activities and findings in electronic laboratory notebook and reagent registries in keeping with the highest industry standards.

Required Qualifications and Experience

·Minimum of a BA/BS or equivalent degree in biology, biochemistry, bioengineering, or a related discipline with 1-4 years of relevant research experience. Title will be based on education, skills and experience level.

·Strong molecular biology skills including Gibson Assembly, Gateway and conventional cloning methods, knowledge in plasmid construct design, agarose gel electrophoresis, DNA purification, plasmid preparation, primer design, and DNA sequence analysis.

·Experience with Next Generation Sequencing (NGS) library preparation for RNA-seq, DNA-seq, 10x Genomics assays.

·Extensive experience with molecular biology software such as SnapGene or Geneious.

·Experience with plasmid transfection of cultured mammalian cells.

·Experience with genomic DNA and RNA purification from various mammalian cell lines and primary cells.

·Passion for organization and experience with sample tracking and maintenance of reagent registries

·Excellent analytical, organizational, communication, and time management skills.

Preferred Qualifications:

·Extensive experience in developing molecular assays such as RT-PCR, digital PCR, and ELISA

·Experience in transduction of mammalian cells with AAV and/or lentivirus

·Experience with multicolor flow cytometry, FACS and fluorescence microscopy

Zag Bio values the diversity of our workforce and maintains a deeply respectful, values-driven working environment. We are committed to equal employment opportunities and non-discrimination for all employees and qualified applicants without regard to a person's race, color, gender, age, religion, national origin, ancestry, disability, veteran status, genetic information, sexual orientation, or any characteristic protected under applicable law.

We are not accepting unsolicited resumes from employment agencies for any employment opportunities at this time.