Research Assistant I - Genomic Analysis Laboratory, Dr. Ecker

The Research Assistant I position will be working as part of a team with the goal of using epigenomics to create an atlas of the human brain at single-cell resolution, through both sequencing and imaging. This position will involve training in specialized techniques related to the Ecker lab focus of single-cell multiomics. Main duties for this position may include: large-scale generation of methylation sequencing libraries using a robotic pipetting system, Cryostat sectioning and fluorescence imaging for spatial transcriptomics through MERSCOPE, nuclei production and sorting, and treatment of nuclei to evaluate chromatin conformation. Other duties include general laboratory maintenance, coordination with collaborators and FACS core for cell sorting, participation in regular team meetings to discuss progress, and well-organized LIMS and data recording.
Essential Functions
(90%) Large-Scale Molecular Biology Techniques

• Chromatin assays
• Bisulfite sequencing
• Perform tissue sectioning with Cryostat
• Isolate cellular nuclei from brain tissue
• Fluorescence staining protocols for imaging
• Magnetic bead DNA purification
• PCR

(10%) Lab maintenance and organization

• General lab/equipment upkeep
• Attend weekly lab meetings and assist with problem solving
• Sample tracking and scheduling
• Updating LIMS as necessary with sample information
• Coordination with others for sample processing

Experience
Required:

• No professional experience required with relevant degree

Preferred

• Experience with robotic liquid handling like Biomek or Tecan
• 0-2 years of experience as a lab technician or equivalent related experience.
• Familiar with molecular biology techniques such as PCR, next-generation sequencing (NGS) library preps, chromatin assays, ATAC-seq, bisulfite sequencing

EDUCATION
Required

• Bachelor's degree in Biological Sciences or related scientific discipline required. Bachelor's degrees in a different discipline and a minimum of 2 years of experience in a scientific role will be considered in lieu.

Preferred

• None

Required
SKILLS AND ABILITIES

• Proven ability to work both independently and also as part of a collaborative team.
• Highly organized, effective at multitasking, adept to changing priorities
• Skill in the operation and maintenance of various lab equipment.
• Ability to learn and carry out new experimental procedures with accuracy and efficiency. Demonstrated ability to follow verbal and/or written protocols, including follow-up on previously begun procedures.
• Demonstrated knowledge and experience in researching information and analyzing data to arrive at valid conclusions.
• Demonstrated ability to record data accurately and enter into appropriate medium.
• Strong interpersonal skills, including tact, diplomacy, and flexibility.
• Excellent communication skills, both oral and written.
• Demonstrated knowledge and experience with both PC and MAC operating systems, standard business office equipment and commonly used software and tools, such as Microsoft Office, electronic mail, and the internet.
• Demonstrated ability to interact effectively with diverse groups. Demonstrated skill in establishing and maintaining effective working relationships with personnel at all levels.
• Theoretical and hands-on knowledge of molecular biology techniques.
• Proficiency with common office software applications as well as software applications used for research, recording, and analysis.

Preferred

• None

The salary range for this position is $18.02 - $21.00 an hour.
SPECIAL CONDITIONS OF EMPLOYMENT

• Must be willing to work a flexible schedule to support research needs.
• Must be willing to work in a human/animal-related research environment.
• Successful completion of the Institute’s background investigation.

Physical Requirements/Mental Activities/Environmental Conditions
The incumbent in this position will be constantly adjusting focus, grasping, hearing, seeing, talking, analyzing, calculating, communicating, reading, writing, and working indoors.