Scientist Iii Jobs

One of the largest Pharmaceutical companies in the world with one purpose: to chase the miracles of science to improve people's lives. They are on course to transform the practice of medicine through breakthrough science and make a positive impact on our people and the communities they serve.

Description

Description

The upstream process development associate within the Manufacturing Technology (MTech) function of Manufacturing and Supply Vaccines (MSV) performs experiments within the upstream cell culture and bacterial group using baculovirus insect cell expression and meningococcus systems. Experiments will be performed at small-scale using flasks and 2L to 30L glass and stainless-steel bioreactors, as well as 200L single use bioreactors using batch and/or fed-batch processes. The candidate is also expected to assist cell culture and bacterial strategies and approaches to increase the yield of vaccine products. The mission of the MTech function is to deliver robust and efficient processes with associated knowledge to transfer to manufacturing.

KEY SKILLS

Operating small-scale (2L-30L) and large-scale (200L) bioreactors

Experiences WITH bacterial meningococcus system

Protein expression in a baculovirus insect cells system

RESPONSIBILITIES

• Perform protein expression in a baculovirus insect cells system using shake flasks and 3-10L glass bioreactors or 200L single-use bioreactors.

• Perform polymer expression in a bacterial meningococcus system using shake flasks and 2-30L glass and stainless-steel bioreactors.

• Perform analytical test, included but not limited to, SDS-PAGE, Western Blot, Culture Plates, Plaque Purification, Microscopy, and downstream purification methods.

• Execute assembly, sterilizing, setup, and cleaning tasks for both glass and stainless-steel bioreactors, while maintain aseptic techniques and practices.

• Document experiments and maintain proper records electronically and in lab notebooks.

• Communicate with co-workers and present upstream results in group and department meetings on a regular basis.

• Contribute to lab organization, maintenance of instruments, and ordering.

• Ensure that work performed is conducted in a safe and compliant manner in adherence with SOPs and protocols.

• Performs other functions as required or assigned and comply with all company policies and standards.

EDUCATION / REQUIREMENTS

Basic Qualifications

• Academic degree in a field of Biology, Microbiology, Chemical Engineering, Chemistry, Biochemistry, Engineering, or related disciplines. Proportional experience is a valid substitute for academic degree.

Preferred Qualifications

• Experience in cell culture procedures and associated aseptic techniques is highly desirable.

• Experience in bacterial procedures and associated BSL-2 aseptic techniques is highly desirable.

• Experience with protein and polymer production in shake flasks and bioreactors.

• Experience with setting up and operating small-scale (2L-30L) and large-scale (200L) bioreactors.

• Ability to work within team environment and self-motivated is preferred.

• Strong written and verbal communication skills.

• Detail-oriented and ability to prioritize assignments.

• Ability to work with limited supervision.

• Fluent in both written and spoken English.