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Research Professional Specialist Jobs
Company | University of Chicago |
Address | Chicago, IL, United States |
Employment type | OTHER |
Salary | |
Category | Higher Education |
Expires | 2023-09-03 |
Posted at | 9 months ago |
Department
- Collaborates with synthetic chemists and computational biologists both at the University and with third-party CROs to improve the affinity and physical properties of small molecules lead compounds.
- Expression of target proteins using E.coli, yeast, SF9, and HEK293F systems.
- Trains new laboratory personnel.
- Mentors and manages the research activities of post-docs, graduate students, research associates and undergraduate students to achieve research objectives.
- Use standard tools of molecular biology to generate protein expression constructs for downstream in vitro expression and cell biology experiments.
- Serves as a resource for collecting data and performing analysis. Contributes to facilitating and promoting a research project by providing scientific or intellectual information.
- Maintaining mammalian cell culture and generating/selecting new cell lines using standard methods including lentiviral transduction and CRISPR knock out/knock.
- Performs other related work as needed.
- Characterization of recombinant proteins using biophysical tools including DSF, ITC, SPR, FP, SEC, CD, and ELISA for subsequent biophysical analysis.
- Develops and executes target specific enzyme-activity and cell-based assays (including high-throughput assays) to elucidate protein function and identify small molecule inhibitors, nanoparticles, and small-molecule conjugates.
- Purification of recombinant proteins using affinity, ion exchange, gel filtration, and other chromatography methods as implemented on an AKTA system.
- Reviews laboratory protocols and training on new techniques. Manage complex data sets for research.
- Translates knowledge of project-relevant scientific and patent literature into new therapeutic opportunities and research processes.
- Leads the contributions to scientific writing and publications, including protocols and grants.
- Mammalian cell culture experience including transfection, viral transduction and CRISPR.
- Demonstrated ability to execute experiments, analyze data, and report conclusions.
- Experience in protein purification methods to support in vitro assay development and characterization by biophysical technologies.
- Experience developing enzymatic and cell-based assays to study protein function and support small-molecule drug discovery.
- Experience in molecular biology methods including cloning, qPCR, RNAseq, and Western Blot.
- Must be flexible and willing to work across multiple projects.
- Self-motivated and curious with a desire to learn from and teach others.
- Ability to thrive in a highly collaborative, fast-paced, team-oriented environment with colleagues from diverse disciplines.
- Have a quantitative mindset and a strong scientific intuition.
- Wet lab environment.
- Resume (required)
- Cover Letter (preferred)
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