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Associate Scientist I, Car Screening
Company | Johnson & Johnson |
Address | Spring House, PA, United States |
Employment type | FULL_TIME |
Salary | |
Category | Hospitals and Health Care |
Expires | 2023-09-09 |
Posted at | 8 months ago |
- Design and complete experiments based on protocols.
- Master’s degree in Life Sciences (e.g. Biology, Biotechnology) or a related field with 1-2 years of academic and/or industry experience is required.
- Maintain a detailed, accurate, complete, and up-to-date laboratory notebook
- Coordinate experimental activities with other team members to deliver results that meet pre-defined timelines.
- Assist in CAR T cell generation, expansion, characterization, and cryopreservation.
- Complete multi color immune cell phenotyping and FACS analysis.
- Data generation, analysis, maintenance, and communication to immediate team and cross-functional teams.
- Thaw, culture and maintain a variety of mammalian cell lines using aseptic technique.
- Analyze and interpret experimental data.
- Highly self-motivated with excellent communication and scientific problem-solving skills is required.
- Experience with in vitro cell culture: aseptic technique, cell-line culturing, freezing and thaw is required.
- Ability to independently perform lab-based responsibilities with limited guidance is required.
- Ability to collaborate with others to plan experiments and coordinate frequent hand-offs of cell lines and data packages to members of other teams is required.
- Excellent record keeping and documentation skills as well as experience using ELN systems is a plus.
- Willingness to think creatively, be flexible and able to work in a fast paced, changing environment is highly desired.
- Experience with multi-color flow cytometry (8-10 colors) and downstream data processing/analysis (FlowJo/Prism) is preferred.
- Experience working with immune cell types such as murine or human T cells is preferred, and any experience with NK cells or Gamma Delta T cells is a plus.
- Experience with functional characterization such as cytotoxicity assays (Incucyte), proliferation assays and cytokine measurements through MSD is preferred.
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