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Research Scientist Protein Biochemist

Company

Dartmouth College
Address , Hanover, Nh
Employment type FULL_TIME
Salary
Expires 2023-06-24
Posted at 1 year ago
Job Description

Position Purpose
The Dartmouth Institute for Biomolecular Targeting (bioMT) is seeking an experienced staff member to join its Core Facilities (Molecular Tools Core and Molecular Interactions and Imaging Core). The successful applicant will possess knowledge and hands-on experience on the design, production, and biophysical characterization of recombinant proteins. The Protein Biochemist will employ high-throughput approaches for construct design and protein expression and develop purification workflows involving multiple chromatographic methods (e.g., AP, IEC, SEC). Previous experience with structural analysis (x-ray, or cryoEM) of proteins and/or in proteomics will be highly desirable. This person will actively contribute to and facilitate multidisciplinary research projects across the Dartmouth community. Other activities include but are not limited to, data collection and reporting, equipment maintenance, and training of bioMT investigators.

Description

Required Qualifications - Education and Yrs Exp
PhD or equivalent combination of education and experience

Required Qualifications - Skills, Knowledge and Abilities
  • Willingness to work with hazardous materials including biological agents and/or hazardous chemicals.
  • Experience with protein purification methods and equipment.
  • Ability to multitask.
  • Ability to draft clear technical protocols.
  • Willingness to work closely with diverse groups of students and research staff, including the ability to manage and resolve conflicts.
  • Ability to learn, develop, and apply new techniques.
  • High competency in basic laboratory skills (pipetting, weighing, sterile technique, and recordkeeping), molecular cloning, and one or more of bacterial, insect, or mammalian cell culture.
  • Ability to work independently and apply critical thinking and sound judgment.
  • Knowledge of computers and software applications.
  • Excellent communication skills.
  • Good organizational and time management skills.

Preferred Qualifications
Four+ years of relevant research experience or the equivalent work in a laboratory is highly desirable

Department Contact for Recruitment Inquiries
Shaniqua Jones

Department Contact Phone Number

Department Contact for Cover Letter and Title
Andreia Verissimo

Department Contact's Phone Number

Equal Opportunity Employer
Dartmouth College is an equal opportunity/affirmative action employer with a strong commitment to diversity and inclusion. We prohibit discrimination on the basis of race, color, religion, sex, age, national origin, sexual orientation, gender identity or expression, disability, veteran status, marital status, or any other legally protected status. Applications by members of all underrepresented groups are encouraged.

Background Check
Employment in this position is contingent upon consent to and successful completion of a pre-employment background check, which may include a criminal background check, reference checks, verification of work history, and verification of any required academic credentials, licenses, and/or certifications, with results acceptable to Dartmouth College. A criminal conviction will not automatically disqualify an applicant from employment. Background check information will be used in a confidential, non-discriminatory manner consistent with state and federal law.

Is driving a vehicle (e.g. Dartmouth vehicle or off road vehicle, rental car, personal car) an essential function of this job?
Not an essential function

Special Instructions to Applicants
This position is funded by a grant. Continuation is based upon available funds.
Dartmouth College has a Tobacco-Free Policy. Smoking and the use of tobacco-based products (including smokeless tobacco) are prohibited in all facilities, grounds, vehicles or other areas owned, operated or occupied by Dartmouth College with no exceptions. For details, please see our policy. https://policies.dartmouth.edu/policy/tobacco-free-policy

Additional Instructions

Key Accountabilities

Description
Research/Experiments
  • As needed, supports eukaryotic cell culture services, including culture maintenance and transfection.
  • Independently optimizes expression conditions and develops purification protocols for diverse protein targets.
  • Troubleshoots and recommends solutions to problems associated with experimental design, procedures, and equipment.
  • Independently designs optimal constructs for protein production in E. coli, HEK293, and Sf9/Hi5 cells.
  • Prepare and optimize protein samples for structure determination by cryoEM to support structural biology research projects.
  • Develops proficiency in laboratory equipment for protein expression, purification, and characterization, including AKTA systems, centrifuges, and a variety of analytical instruments.
  • Works collaboratively with core facility staff, faculty, scientists, and graduate students from across the Dartmouth community to express and purify recombinant proteins for use in research projects.
  • Gains experience with diverse biochemical and biophysical techniques, including SPR, ITC, Fluorescence polarization and X-ray Crystallography.
  • Supports proteomics projects by helping with sample preparation, LC-MS/MS applications, and data analysis.

Percentage Of Time
65

Description
Data Collection/Preparation
  • Performs literature searches to develop information of relevance to current projects.
  • Collects, analyzes, and interprets data resulting from expression and purification experiments.
  • Prepares appropriate and understandable representations of data such as sequence files, graphs, charts, etc.
  • Participates in the preparation of annual reports.
  • Contributes to the preparation of scientific manuscripts by drafting portions of the text, preparing graphs, tables, and charts, etc. as needed.
  • Presents data at laboratory meetings, departmental retreats, and scientific conferences.

Percentage Of Time
15%

Description
Laboratory
  • Adheres to laboratory safety requirements including general, biological, and chemical/hazardous waste safety disposal methods.
  • Develops and tracks quality-assurance and quality-control metrics.
  • Develops SOPs and other documentation for common procedures and methods.
  • Maintains hardcopy and computerized records associated with laboratory activities.

Percentage Of Time
10%

Description
Training
  • Develops instructional materials as needed.
  • Provides training in laboratory methods, procedures, and techniques to multiple levels of scientific investigators (graduate students, post-doctoral fellows, staff scientists and faculty).

Percentage Of Time
10%

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Demonstrates a commitment to diversity, inclusion, and cultural awareness through actions, interactions, and communications with others.
-
Performs other duties as assigned.